wcm.04.2025.798.804

The literature focuses on the potential of ecoenzymes as biocatalysts in wastewater treatment. The strategy used is Systematic Literature Review as an instrument. Metadata was collected using Publish or Perish, and 3.151 articles were extracted from CrossCef, PubMed, OpenAlex, and Google Scholar during the period 2015- 2025. After eliminating duplicate and anonymous articles, 1.898 articles remained, then bibliometric as data processor which is analyzed by VOSviewer software. This literature study presents a more accurate bibliographical objective for future researchers according to the themes discussed. The potential of ecoenzymes as biocatalysts in wastewater treatment lies in their enzyme content, such as protease, lipase, and amylase. These enzymes play an important role in the degradation of organic pollutants contained in wastewater. In addition, the organic acid and the presence of lactic acid bacteria have been found to inhibit the growth of pathogenic bacteria commonly found in wastewater, such as Staphylococcus aureus and Escherichia coli. Although they have the potential to be developed as biocatalysts, the application of ecoenzymes in conjunction with existing wastewater treatment technologies such as biofilters, constructed wetlands, and combinations with phytoremediation still face challenges, particularly related to enzyme stability in field conditions. Immobilized enzymes have been proven to be one of the promising methods to enhance enzyme stability. Further studies are needed in scale-up enzyme-based biocatalysts and integration with established systems