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ISTN Lake is located in a campus environment and has an important ecological role as a water source, local conservation area, and natural laboratory for academic research. However, increasing pollution pressure from domestic activities around the lake can cause a decline in water quality and disrupt its ecological function. Therefore, a scientific study is needed to assess the lake’s water quality, identify pollution sources, and formulate waste management strategies that support the sustainability of the lake ecosystem and its use as a natural laboratory. This study uses a quantitative descriptive approach, with measurements of physicochemical parameters (pH, DO, BOD, COD, TSS, TN, TP), and water quality index (IKA) analysis to assess the level of pollution. The results of the study showed that most observation points had water quality status in the “MODERATE” category (IKA: 67.76 – 73.89), with one point reaching the “GOOD” category (IKA: 77.63). The main factors affecting water quality come from high organic loads due to domestic waste and nutrient runoff from the surrounding environment. The analysis shows that a better waste management system is needed, restoration of riverbank vegetation, and regular water quality monitoring are needed to maintain the sustainability of the lake. ISTN lake management can adopt an integrated and sustainable approach, including pollution control, ecosystem restoration, and utilization of the lake as an ecological laboratory. By implementing an effective waste management system, technology-based water quality monitoring, and environmental education for the academic community, the lake can continue to function as a healthy water resource and support the campus ecosystem.